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Image Search Results
Journal: Cell reports
Article Title: A Systematic Protein Turnover Map for Decoding Protein Degradation
doi: 10.1016/j.celrep.2020.108378
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP Abcam Cat# ab290; RRID: AB_303395 Rabbit polyclonal anti-Rpn1 Dan Finley N/A Rabbit polyclonal anti-Rpn5 Dan Finley ( Shi et al., 2016 ) N/A Rabbit polyclonal anti-Rpn8 Dan Finley N/A Rabbit polyclonal anti-Rpn12 Dan Finley N/A Mouse anti-IgG kappa binding protein-HRP Santa Cruz Biotechnology Cat# sc-516102; RRID: AB_2687626 Mouse monoclonal anti-rabbit IgG-HRP Santa Cruz Biotechnology Cat# sc-2357; RRID: AB_628497 Mouse monoclonal anti-Pgk1 Invitrogen Cat# 459250; RRID: AB_2532235 Mouse monoclonal anti-FLAG Sigma-Aldrich Cat# F1804; RRID AB_262044 Chemicals, Peptides, and Recombinant Proteins heavy [ 13 C 6 / 15 N 2 ] L-lysine Cambridge Isotope laboratories, Inc. CNLM-291-H-PK Drop-out Mix Minus Lysine w/o Yeast Nitrogen Base US Biological Cat# D9515B Critical Commercial Assays SuperSignal West Pico SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 SuperSignal West Pico Thermo Fisher Scientific Cat# 34580 SuperSignal West Dura SuperSignal West Dura Thermo Fisher Scientific Cat# 34076 SuperSignal West Dura Thermo Fisher Scientific Cat# 34076 Deposited Data Mass spectrometry proteomics data Proteomics Identifications Database (PRIDE) repository PRIDE: PXD008706 Experimental Models: Yeast strains ( Saccharomyces cerevisiae ) BY4743 Dharmacon N/A BY4741 Dharmacon N/A BY4741; pep4Δ::kanMX Dharmacon N/A BY4741; CHS3-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; pep4Δ::kanMX CHS3-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; FCY2-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; pep4Δ::kanMX FCY2-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; CPR4-TEV-6xGly-3xFLAG::hphMX This study N/A BY4741; pep4Δ::kanMX CPR4-TEV-6xGly-3xFLAG::hphMX This study N/A Recombinant DNA Plasmid: pTF272 (TEV-6xGly-3xFLAG) Tim Formosa
Techniques: Binding Assay, Recombinant, Mass Spectrometry, Plasmid Preparation, Software